rabbit anti-syn1 Search Results


syp  (Cusabio)
92
Cusabio syp
Syp, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syp/product/Cusabio
Average 92 stars, based on 1 article reviews
syp - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
anti synapsin 1  (Cusabio)
93
Cusabio anti synapsin 1
Anti Synapsin 1, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti synapsin 1/product/Cusabio
Average 93 stars, based on 1 article reviews
anti synapsin 1 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
rabbit anti-syn1  (Bioconnect Systems Inc)
90
Bioconnect Systems Inc rabbit anti-syn1
Synaptic phenotype in KdVS patient derived iNeurons. (A) Schematic presentation of the protocol to colocalize LC3 and <t>SYN1-SYN2.</t> Image of a dendrite of a control iNeuron at DIV21 after overnight incubation without B27 and treated with 200 nM BAF for 10 min before fixation. Scale bar: 10 µm. (B) Representative images showing dendrites stained for MAP2 and <t>SYN1-SYN2</t> and SYN1-SYN2 puncta quantification at DIV21 for all lines. n = 60 for C 1 ; n = 57 for KdVS 1 ; n = 24 for CRISPR 1 ; n = 15 for C 2 ; n = 20 KdVS 2 ; n = 34 for KdVS 3 . Scale bar: 20 µm. One-way ANOVA and Sidak’s multiple comparison test were used to test for statistically significant differences. (C) Representative voltage clamp recordings at V h = −60 mV showing sEPSCs at DIV21. (D) sEPSC amplitude and (E) frequency quantification. n = 9 for C 1 ; n = 11 for KdVS 1 ; n = 10 for CRISPR 1 ; n = 15 for C 2 ; n = 8 for KdVS 2 and KdVS 3 (obtained in two independent experiments). (F) Schematic representation for neuronal network measurements on MEAs (3 min of recording). Representative raster plots for C 1 , KdVS 1 and CRISPR 1 derived networks that were plated at similar high densities, measured at DIV 30. (G) Quantification of the mean firing rate and (H) network burst rate, (I) percentage of random spikes, and (J) coefficient of variation (CV) calculated on the inter network burst interval. n = 15 for C 1 ; n = 18 for KdVS 1 ; n = 16 for CRISPR 1 . If not stated differently, data presented in this figure were obtained in at least 3 independent experiments and statistically significant differences were tested through Kruskal-Wallis and Dunn’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.0001.
Rabbit Anti Syn1, supplied by Bioconnect Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-syn1/product/Bioconnect Systems Inc
Average 90 stars, based on 1 article reviews
rabbit anti-syn1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit anti-syn-1  (Huabio Inc)
90
Huabio Inc rabbit anti-syn-1
Synaptic phenotype in KdVS patient derived iNeurons. (A) Schematic presentation of the protocol to colocalize LC3 and <t>SYN1-SYN2.</t> Image of a dendrite of a control iNeuron at DIV21 after overnight incubation without B27 and treated with 200 nM BAF for 10 min before fixation. Scale bar: 10 µm. (B) Representative images showing dendrites stained for MAP2 and <t>SYN1-SYN2</t> and SYN1-SYN2 puncta quantification at DIV21 for all lines. n = 60 for C 1 ; n = 57 for KdVS 1 ; n = 24 for CRISPR 1 ; n = 15 for C 2 ; n = 20 KdVS 2 ; n = 34 for KdVS 3 . Scale bar: 20 µm. One-way ANOVA and Sidak’s multiple comparison test were used to test for statistically significant differences. (C) Representative voltage clamp recordings at V h = −60 mV showing sEPSCs at DIV21. (D) sEPSC amplitude and (E) frequency quantification. n = 9 for C 1 ; n = 11 for KdVS 1 ; n = 10 for CRISPR 1 ; n = 15 for C 2 ; n = 8 for KdVS 2 and KdVS 3 (obtained in two independent experiments). (F) Schematic representation for neuronal network measurements on MEAs (3 min of recording). Representative raster plots for C 1 , KdVS 1 and CRISPR 1 derived networks that were plated at similar high densities, measured at DIV 30. (G) Quantification of the mean firing rate and (H) network burst rate, (I) percentage of random spikes, and (J) coefficient of variation (CV) calculated on the inter network burst interval. n = 15 for C 1 ; n = 18 for KdVS 1 ; n = 16 for CRISPR 1 . If not stated differently, data presented in this figure were obtained in at least 3 independent experiments and statistically significant differences were tested through Kruskal-Wallis and Dunn’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.0001.
Rabbit Anti Syn 1, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-syn-1/product/Huabio Inc
Average 90 stars, based on 1 article reviews
rabbit anti-syn-1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Synaptic phenotype in KdVS patient derived iNeurons. (A) Schematic presentation of the protocol to colocalize LC3 and SYN1-SYN2. Image of a dendrite of a control iNeuron at DIV21 after overnight incubation without B27 and treated with 200 nM BAF for 10 min before fixation. Scale bar: 10 µm. (B) Representative images showing dendrites stained for MAP2 and SYN1-SYN2 and SYN1-SYN2 puncta quantification at DIV21 for all lines. n = 60 for C 1 ; n = 57 for KdVS 1 ; n = 24 for CRISPR 1 ; n = 15 for C 2 ; n = 20 KdVS 2 ; n = 34 for KdVS 3 . Scale bar: 20 µm. One-way ANOVA and Sidak’s multiple comparison test were used to test for statistically significant differences. (C) Representative voltage clamp recordings at V h = −60 mV showing sEPSCs at DIV21. (D) sEPSC amplitude and (E) frequency quantification. n = 9 for C 1 ; n = 11 for KdVS 1 ; n = 10 for CRISPR 1 ; n = 15 for C 2 ; n = 8 for KdVS 2 and KdVS 3 (obtained in two independent experiments). (F) Schematic representation for neuronal network measurements on MEAs (3 min of recording). Representative raster plots for C 1 , KdVS 1 and CRISPR 1 derived networks that were plated at similar high densities, measured at DIV 30. (G) Quantification of the mean firing rate and (H) network burst rate, (I) percentage of random spikes, and (J) coefficient of variation (CV) calculated on the inter network burst interval. n = 15 for C 1 ; n = 18 for KdVS 1 ; n = 16 for CRISPR 1 . If not stated differently, data presented in this figure were obtained in at least 3 independent experiments and statistically significant differences were tested through Kruskal-Wallis and Dunn’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.0001.

Journal: Autophagy

Article Title: Imbalanced autophagy causes synaptic deficits in a human model for neurodevelopmental disorders

doi: 10.1080/15548627.2021.1936777

Figure Lengend Snippet: Synaptic phenotype in KdVS patient derived iNeurons. (A) Schematic presentation of the protocol to colocalize LC3 and SYN1-SYN2. Image of a dendrite of a control iNeuron at DIV21 after overnight incubation without B27 and treated with 200 nM BAF for 10 min before fixation. Scale bar: 10 µm. (B) Representative images showing dendrites stained for MAP2 and SYN1-SYN2 and SYN1-SYN2 puncta quantification at DIV21 for all lines. n = 60 for C 1 ; n = 57 for KdVS 1 ; n = 24 for CRISPR 1 ; n = 15 for C 2 ; n = 20 KdVS 2 ; n = 34 for KdVS 3 . Scale bar: 20 µm. One-way ANOVA and Sidak’s multiple comparison test were used to test for statistically significant differences. (C) Representative voltage clamp recordings at V h = −60 mV showing sEPSCs at DIV21. (D) sEPSC amplitude and (E) frequency quantification. n = 9 for C 1 ; n = 11 for KdVS 1 ; n = 10 for CRISPR 1 ; n = 15 for C 2 ; n = 8 for KdVS 2 and KdVS 3 (obtained in two independent experiments). (F) Schematic representation for neuronal network measurements on MEAs (3 min of recording). Representative raster plots for C 1 , KdVS 1 and CRISPR 1 derived networks that were plated at similar high densities, measured at DIV 30. (G) Quantification of the mean firing rate and (H) network burst rate, (I) percentage of random spikes, and (J) coefficient of variation (CV) calculated on the inter network burst interval. n = 15 for C 1 ; n = 18 for KdVS 1 ; n = 16 for CRISPR 1 . If not stated differently, data presented in this figure were obtained in at least 3 independent experiments and statistically significant differences were tested through Kruskal-Wallis and Dunn’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.0001.

Article Snippet: Used primary antibodies were: rabbit anti-KANSL1 (1:500; Sigma-Aldrich, HPA006874); mouse anti-MAP2 (1:1000; Sigma-Aldrich, M4403); guinea pig anti-MAP2 (1:1000; Synaptic Systems, 188004); guinea pig anti-SYN1-SYN2 (1:1000; Synaptic Systems, 106004); rabbit anti-SYN1 (1:500; BioConnect, AB1543P); mouse anti-HOMER1 (1:500; Synaptic Systems, 160,011); rabbit anti-DLG4/PSD95 (1:50; Cell Signaling Technology, D27E11); mouse anti-pan axon (1:1000; Covance, SMI-312 R); rabbit anti-SQSTM1 (1:500; Sigma-Aldrich, P0067); mouse anti-LC3 (1:500; NanoTools, 0231–100/LC3-5 F10); rabbit anti-LAMP1 (1:200; Sigma-Aldrich, L1418-200ul); mouse anti-8-oxo-dG (1:100; R&D Systems, 4354-MC-050), rabbit anti-H4K16ac (1:400; Abcam, ab109463), rabbit anti-NANOG (1:100; Abcam, ab21624), mouse anti-SSEA4 (1:50; Abcam, ab16287), rabbit anti-POU5F1(1:250; Abcam, ab19857), mouse anti-TRA1-81 (1:100; Millipore, MAB4381) .

Techniques: Derivative Assay, Incubation, Staining, CRISPR

Apocynin treatment rescues synaptic phenotype. (A) Representative images of 8-oxo-dG stainings and 8-oxo-dG quantification for untreated and APO treated iNeurons relative to respective untreated control cells at DIV21. n = 39 for C 1 ; n = 46 for C 1 + APO; n = 42 for KdVS 1 ; n = 51 for KdVS 1 + APO; n = 26 for CRISPR 1 ; n = 25 for CRISPR 1 + APO; n = 26 for C 2 ; n = 25 for C 2 + APO; n = 28 for KdVS 2 ; n = 27 for KdVS 2 + APO; n = 31 for KdVS 3 ; n = 23 for KdVS 3 + APO. Scale bar: 20 µm. (B) Representative images of SQSTM1 stainings of iNeurons at DIV21 and fluorescence quantification in untreated and APO treated iNeurons relative to untreated control cells at DIV21. n = 43 for C 1 ; n = 34 for C 1 + APO; n = 34 for KdVS 1 ; n = 38 for KdVS1+ APO; n = 40 for CRISPR 1 ; n = 39 for CRISPR 1 + APO; n = 16 for C 2 ; n = 19 for C 2 + APO; n = 25 for KdVS 2 ; n = 27 for KdVS 2 + APO; n = 34 for KdVS 3 ; n = 24 for KdVS 3 + APO. Scale bar: 20 µm. (C) Representative images of dendrites stained for MAP2 and SYN1-SYN2 for C 1 , KdVS 1 and CRISPR 1 at DIV21 either untreated or APO treated and SYN1-SYN2 quantification. n = 11 for C 1 ; n = 9 for C 1 + APO; n = 12 for KdVS 1 ; KdVS 1 + APO; n = 10 for CRISPR 1 ; CRISPR 1 + APO; n = 7 for C 2 ; C 2 + APO; KdVS 2 ; KdVS 2 + APO; KdVS 3 , KdVS 3 + APO. Scale bar: 20 µm. Two-way ANOVA was used to determine statistically significant changes. (D) Representative voltage clamp recordings at V h = −60 mV showing sEPSCs at DIV21 with and without APO treatment during differentiation. (E) sEPSC frequency and (F) amplitude quantifications. n = 8 for C 1 ; C 1 + APO; n = 9 for KdVS 1 ; KdVS1+ APO; n = 11 for CRISPR 1 ; n = 8 for CRISPR 1 + APO; n = 15 for C 2 ; n = 14 for C 2 + APO; n = 8 for KdVS 2 ; n = 9 for KdVS 2 + APO; n = 8 for KdVS 3 ; KdVS 3 + APO. (G) Representative raster plots for untreated C 1 network and untreated and APO treated CRISPR 1 network at DIV30 (3 min. of recording). Quantification of (H) mean firing rate, (I) network burst frequency, (J) percentage of random spikes, and (K) CV of inter-network burst interval. n = 15 for C 1 ; n = 17 for C 1 + APO; n = 16 for CRISPR 1 ; n = 15 for CRISPR 1 + APO. All data presented in this figure were generated in at least 2 independent experiments and statistically significant differences were tested through Kruskal-Wallis and Dunn’s multiple comparison test, if not mentioned differently. All samples were always compared to the respective untreated control; only significant differences were indicated. * P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.0001.

Journal: Autophagy

Article Title: Imbalanced autophagy causes synaptic deficits in a human model for neurodevelopmental disorders

doi: 10.1080/15548627.2021.1936777

Figure Lengend Snippet: Apocynin treatment rescues synaptic phenotype. (A) Representative images of 8-oxo-dG stainings and 8-oxo-dG quantification for untreated and APO treated iNeurons relative to respective untreated control cells at DIV21. n = 39 for C 1 ; n = 46 for C 1 + APO; n = 42 for KdVS 1 ; n = 51 for KdVS 1 + APO; n = 26 for CRISPR 1 ; n = 25 for CRISPR 1 + APO; n = 26 for C 2 ; n = 25 for C 2 + APO; n = 28 for KdVS 2 ; n = 27 for KdVS 2 + APO; n = 31 for KdVS 3 ; n = 23 for KdVS 3 + APO. Scale bar: 20 µm. (B) Representative images of SQSTM1 stainings of iNeurons at DIV21 and fluorescence quantification in untreated and APO treated iNeurons relative to untreated control cells at DIV21. n = 43 for C 1 ; n = 34 for C 1 + APO; n = 34 for KdVS 1 ; n = 38 for KdVS1+ APO; n = 40 for CRISPR 1 ; n = 39 for CRISPR 1 + APO; n = 16 for C 2 ; n = 19 for C 2 + APO; n = 25 for KdVS 2 ; n = 27 for KdVS 2 + APO; n = 34 for KdVS 3 ; n = 24 for KdVS 3 + APO. Scale bar: 20 µm. (C) Representative images of dendrites stained for MAP2 and SYN1-SYN2 for C 1 , KdVS 1 and CRISPR 1 at DIV21 either untreated or APO treated and SYN1-SYN2 quantification. n = 11 for C 1 ; n = 9 for C 1 + APO; n = 12 for KdVS 1 ; KdVS 1 + APO; n = 10 for CRISPR 1 ; CRISPR 1 + APO; n = 7 for C 2 ; C 2 + APO; KdVS 2 ; KdVS 2 + APO; KdVS 3 , KdVS 3 + APO. Scale bar: 20 µm. Two-way ANOVA was used to determine statistically significant changes. (D) Representative voltage clamp recordings at V h = −60 mV showing sEPSCs at DIV21 with and without APO treatment during differentiation. (E) sEPSC frequency and (F) amplitude quantifications. n = 8 for C 1 ; C 1 + APO; n = 9 for KdVS 1 ; KdVS1+ APO; n = 11 for CRISPR 1 ; n = 8 for CRISPR 1 + APO; n = 15 for C 2 ; n = 14 for C 2 + APO; n = 8 for KdVS 2 ; n = 9 for KdVS 2 + APO; n = 8 for KdVS 3 ; KdVS 3 + APO. (G) Representative raster plots for untreated C 1 network and untreated and APO treated CRISPR 1 network at DIV30 (3 min. of recording). Quantification of (H) mean firing rate, (I) network burst frequency, (J) percentage of random spikes, and (K) CV of inter-network burst interval. n = 15 for C 1 ; n = 17 for C 1 + APO; n = 16 for CRISPR 1 ; n = 15 for CRISPR 1 + APO. All data presented in this figure were generated in at least 2 independent experiments and statistically significant differences were tested through Kruskal-Wallis and Dunn’s multiple comparison test, if not mentioned differently. All samples were always compared to the respective untreated control; only significant differences were indicated. * P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.0001.

Article Snippet: Used primary antibodies were: rabbit anti-KANSL1 (1:500; Sigma-Aldrich, HPA006874); mouse anti-MAP2 (1:1000; Sigma-Aldrich, M4403); guinea pig anti-MAP2 (1:1000; Synaptic Systems, 188004); guinea pig anti-SYN1-SYN2 (1:1000; Synaptic Systems, 106004); rabbit anti-SYN1 (1:500; BioConnect, AB1543P); mouse anti-HOMER1 (1:500; Synaptic Systems, 160,011); rabbit anti-DLG4/PSD95 (1:50; Cell Signaling Technology, D27E11); mouse anti-pan axon (1:1000; Covance, SMI-312 R); rabbit anti-SQSTM1 (1:500; Sigma-Aldrich, P0067); mouse anti-LC3 (1:500; NanoTools, 0231–100/LC3-5 F10); rabbit anti-LAMP1 (1:200; Sigma-Aldrich, L1418-200ul); mouse anti-8-oxo-dG (1:100; R&D Systems, 4354-MC-050), rabbit anti-H4K16ac (1:400; Abcam, ab109463), rabbit anti-NANOG (1:100; Abcam, ab21624), mouse anti-SSEA4 (1:50; Abcam, ab16287), rabbit anti-POU5F1(1:250; Abcam, ab19857), mouse anti-TRA1-81 (1:100; Millipore, MAB4381) .

Techniques: CRISPR, Fluorescence, Staining, Generated